Details

Autor(en) / Beteiligte

• Han, Dongni
• Goudeau, Bertrand
• Manojlovic, Dragan
• Jiang, Dechen
• Fang, Danjun
• Sojic, Neso

Titel

Electrochemiluminescence Loss in Photobleaching

Ist Teil von

• Angewandte Chemie International Edition, 2021-03, Vol.60 (14), p.7686-7690

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The effects of photobleaching on electrochemiluminescence (ECL) was investigated for the first time. The plasma membrane of Chinese Hamster Ovary (CHO) cells was labeled with a [Ru(bpy)3]2+ derivative. Selected regions of the fixed cells were photobleached using the confocal mode with sequential stepwise illumination or cumulatively and they were imaged by both ECL and photoluminescence (PL). ECL was generated with a model sacrificial coreactant, tri‐n‐propylamine. ECL microscopy of the photobleached regions shows lower ECL emission. We demonstrate a linear correlation between the ECL decrease and the PL loss due to the photobleaching of the labels immobilized on the CHO membranes. The presented strategy provides valuable information on the fundamentals of the ECL excited state and opens new opportunities for exploring cellular membranes by combining ECL microscopy with photobleaching techniques such as fluorescence recovery after photobleaching (FRAP) or fluorescence loss in photobleaching (FLIP) methods. Light versus light! The impact of photobleaching on the electrochemiluminescence was investigated at the level of single cells. Specific regions of the labeled membranes were photobleached confocally and then visualized by multimodal imaging. This study demonstrates the crucial role of the photobleaching process on the electrochemiluminescence signal.