Details

Autor(en) / Beteiligte

• Dong, Yan‐hua
• Zhou, Cheng‐jie
• Zhang, Meng‐yuan
• Tao, Jin
• Zhang, Xiao‐meng
• An, Lu
• Zhang, Ju
• Yang, Jie
• Liu, Dong‐jun
• Cang, Ming

Titel

MiR‐455‐5p monitors myotube morphogenesis by targeting mylip

Ist Teil von

• Journal of cellular biochemistry, 2021-04, Vol.122 (3-4), p.442-455

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• As a posttranscriptional regulatory factor, microRNA (miRNA) plays an important role in the formation of myotubes. However, little is known about the mechanism of miRNA regulating myotube morphogenesis. Here, we aimed to characterize the function of miR‐455‐5p in myotube morphogenesis by inducing differentiation in C2C12 myoblasts containing murine Mylip fragments with the miR‐455‐5p target sequence. We found that miR‐455‐5p overexpression promoted the differentiation and hypertrophy of myotubes, while miR‐455‐5p inhibition led to the failure of myotube differentiation and formation of short myotubes. Furthermore, we demonstrated that miR‐455‐5p directly targeted the Mylip 3’‐untranslated region, which plays a key role in monitoring myotube morphogenesis. Interestingly, the expression and function of Mylip were opposite to those of miR‐455‐5p during myogenesis. Our data uncovered novel miR‐455‐5p targets and established a functional link between Mylip and myotube morphogenesis. Understanding the involvement of Mylip in myotube morphogenesis provides insight into the function of the gene regulatory network. miR‐455‐5p overexpression promoted the differentiation and hypertrophy of myotubes, while miR‐455‐5p inhibition led to the failure of myotube differentiation and formation of short myotubes. miR‐455‐5p directly targeted the Mylip 3’‐untranslated region, which plays a key role in monitoring myotube morphogenesis.