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R2R3-MYB Transcription Factor MdMYB73 Confers Increased Resistance to the Fungal Pathogen Botryosphaeria dothidea in Apples via the Salicylic Acid Pathway
Ist Teil von
Journal of agricultural and food chemistry, 2021-01, Vol.69 (1), p.447-458
Ort / Verlag
United States
Erscheinungsjahr
2021
Quelle
MEDLINE
Beschreibungen/Notizen
MYB transcription factors (TFs) participate in many biological processes. However, the molecular mechanisms by which MYB TFs affect plant resistance to apple ring rot remain poorly understood. Here, the R2R3-MYB gene
was cloned from "
" apples and functionally characterized as a positive regulator of the defense response to
. qRT-PCR and GUS staining demonstrated that
was strongly induced in apple fruits and transgenic calli after inoculation with
.
overexpression improved resistance to
in apple calli and fruits, while
suppression weakened. Increased resistance to
was also observed in
-expressing
. Interestingly, salicylic acid (SA) contents and the expression levels of genes related with SA synthesis and signaling were greater in
-overexpressing plant materials compared to wild-type controls after inoculation, suggesting that MdMYB73 might enhance resistance to
the SA pathway. Finally, we discovered that MdMYB73 interacts with MdWRKY31, a positive regulator of
. Together, MdWRKY31 and MdMYB73 enhanced
resistance in apples. Our results clarify the mechanisms by which MdMYB73 improves resistance to
and suggest that resistance may be affected by regulating the SA pathway.