Details

Autor(en) / Beteiligte

• Romero‐Olmedo, Addi J.
• Schulz, Axel R.
• Huber, Magdalena
• Brehm, Corinna U.
• Chang, Hyun‐Dong
• Chiarolla, Cristina M.
• Bopp, Tobias
• Skevaki, Chrysanthi
• Berberich‐Siebelt, Friederike
• Radbruch, Andreas
• Mei, Henrik E.
• Lohoff, Michael

Titel

Deep phenotypical characterization of human CD3+CD56+ T cells by mass cytometry

Ist Teil von

• European journal of immunology, 2021-03, Vol.51 (3), p.672-681

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• CD56+ T cells are a group of pro‐inflammatory CD3+ lymphocytes with characteristics of natural killer cells, being involved in antimicrobial immune defense. Here, we performed deep phenotypic profiling of CD3+CD56+ cells in peripheral blood of normal human donors and individuals sensitized to birch‐pollen or/and house dust mite by high‐dimensional mass cytometry combined with manual and computational data analysis. A co‐regulation between major conventional T‐cell subsets and their respective CD3+CD56+ cell counterparts appeared restricted to CD8+, MAIT, and TCRγδ+ T‐cell compartments. Interestingly, we find a co‐regulation of several CD3+CD56+ cell subsets in allergic but not in healthy individuals. Moreover, using FlowSOM, we distinguished a variety of CD56+ T‐cell phenotypes demonstrating a hitherto underestimated heterogeneity among these cells. The novel CD3+CD56+ subset description comprises phenotypes superimposed with naive, memory, type 1, 2, and 17 differentiation stages, in part represented by a phenotypical continuum. Frequencies of two out of 19 CD3+CD56+ FlowSOM clusters were significantly diminished in allergic individuals, demonstrating less frequent presence of cells with cytolytic, presumably protective, capacity in these donors consistent with defective expansion or their recruitment to the affected tissue. Our results contribute to defining specific cell populations to be targeted during therapy for allergic conditions. Mass cytometry analysis shows co‐regulated frequency of human CD3+CD56+ and conventional T‐cell subsets. Only during airborne allergy, co‐regulated frequency is also observed between particular CD3+CD56+ cell subsets. FlowSOM analysis reveals CD3+CD56+ cluster heterogeneity previously not appreciated. Two clusters appear diminished in allergy, one positively correlating with specific IgE titers.