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Isolation and structure determination of new linear azole-containing peptides spongiicolazolicins A and B from Streptomyces sp. CWH03
Ist Teil von
Applied microbiology and biotechnology, 2021-01, Vol.105 (1), p.93-104
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2021
Quelle
MEDLINE
Beschreibungen/Notizen
Linear azole-containing peptides are a class of ribosomally synthesized and post-translationally modified peptides. We performed a chemical investigation on marine actinomycetes, and new linear azole-containing peptides named spongiicolazolicins A and B were found in the MeOH extracts of a newly isolated strain
Streptomyces
sp. CWH03 (NBRC 114659) and two strains of
S. spongiicola
(strain HNM0071
T
: DSM 103383
T
and strain 531S: NBRC 113560). The strain
Streptomyces
sp. CWH03 was indicated to be a new species closely related to
S. spongiicola
by phylogenetic analysis using the genome sequence. The new peptides named spongiicolazolicins A and B were isolated from the cell of
Streptomyces
sp. CWH03. The partial structure of spongiicolazolicin A was determined by 2D NMR experiments. Based on data of MS/MS experiments, the chemical structures of spongiicolazolicins A and B were proposed using the amino acid sequence deduced from the precursor-encoding gene, which was found from whole-genome sequence data of
Streptomyces
sp. CWH03. The biosynthetic gene cluster of spongiicolazolicins was proposed based on comparative analysis with that of a known linear azole peptide goadsporin.
Key points
•
Streptomyces sp. CWH03 was a new species isolated from marine sediment.
•
New linear azole-containing peptides named spongiicolazolicins A and B were isolated.
•
Biosynthetic pathway of spongiicolazolicins was proposed.