Details

Autor(en) / Beteiligte

• Kim, Yunsik
• Kim, Young Jin
• Paek, Kyung-Hee
• Sankar, Ramanjulu

Titel

Temperature-specific vsiRNA confers RNAi-mediated viral resistance at elevated temperature in Capsicum annuum

Ist Teil von

• Journal of experimental botany, 2021-02, Vol.72 (4), p.1432-1448

Ort / Verlag

England

Erscheinungsjahr

2021

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Resistance (R) gene-mediated resistance is a robust and efficient antiviral immune system in the plants. Thus, when R-mediated resistance was suppressed at elevated temperatures, resistance towards viruses was expected to be completely collapsed. Nonetheless, the multiplication of Tobacco mosaic virus pathotype P0 (TMV-P0) was inhibited, and TMV-P0 particles were only occasionally present in the systemic leaves of pepper plants (Capsicum annuum). RNAi-mediated RNA silencing is a well-known antiviral immune mechanism. At elevated temperatures, RNAi-mediated antiviral resistance was induced and virus-derived siRNAs (vsiRNAs) were dramatically increased. Through sRNA-sequencing (sRNA-Seq) analysis, we revealed that vsiRNAs derived from TMV-P0 were greatly increased. Intriguingly, virus-infected plants could select the temperature-specific vsiRNAs for antiviral resistance from the amplified vsiRNAs at elevated temperatures. Pre-application of these temperature-specific vsiRNAs endowed antiviral resistance of the plants. Therefore, plants sustain antiviral resistance by activating RNAi-mediated resistance, based on temperature-specific vsiRNAs at elevated temperatures.