The Network of Replication, Transcription, and Reverse Transcription of a Synthetic Genetic Cassette
International ed. in English, 2021
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- The Network of Replication, Transcription, and Reverse Transcription of a Synthetic Genetic Cassette
- Ist Teil von
- Angewandte Chemie International Edition, 2021-02, Vol.60 (8), p.4175-4182
- Auflage
- International ed. in English
- Ort / Verlag
- Germany: Wiley Subscription Services, Inc
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Synthetic nucleic acids, with four non‐canonical nucleobases, can function as genetic materials. A comprehensive analysis of PCR amplification, transcription, reverse transcription, and cloning was done to screen for alternative genetic monomers. A small library of six modified nucleobases was selected: the modified 2′‐deoxyribonucleoside (dZTPs) and ribonucleoside (rZTPs) triphosphates of 7‐deaza‐adenine, 5‐chlorouracil, 7‐deaza‐guanine or inosine together with 5‐fluorocytosine or 5‐bromocytosine. The fragments composed of one to four modified nucleotides (denoted as DZA) have been successfully recognized and transcribed to natural or modified RNA (denoted as RZA) by T7 RNA polymerase. The fully modified RZA fragment could be reverse transcribed and then amplified in the presence of various dZTPs. Noticeably, modified fragments could function as genetic templates in vivo by encoding the 678 base pair gene of a fluorescent protein in bacteria. These results demonstrate the existence of a fully simulated genetic circuit that uses synthetic materials. Alternative genetic information flow encoded in four non‐canonical nucleotides can be carried out by natural biological systems. Natural polymerases can synthesize the entirely morphed DNA (DZA) using a DNA template that, in turn, can serve as a template to produce a fully modified RNA (RZA), and be reverse transcribed back to DZA. In bacteria, various DZA genes can successfully encode for a functional fluorescent protein.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1433-7851eISSN: 1521-3773DOI: 10.1002/anie.202011887Titel-ID: cdi_proquest_miscellaneous_2457668698
- Format
- –
- Schlagworte
- Adenine, Amplification, artificial genes, Bases (nucleic acids), Circuits, Cloning, Cytosine - analogs & derivatives, Cytosine - metabolism, DNA-directed RNA polymerase, DNA-Directed RNA Polymerases - metabolism, Escherichia coli - genetics, Escherichia coli - metabolism, Fluorescence, Fragments, Genetic analysis, Guanine, Guanine - analogs & derivatives, Guanine - metabolism, Monomers, non-canonical nucleotides, Nucleic acids, Nucleic Acids - metabolism, Nucleotides, Plasmids - genetics, Plasmids - metabolism, Polymerase Chain Reaction, Reverse transcription, Ribonucleic acid, RNA, RNA polymerase, synthetic biology, Synthetic Biology - methods, transcription, Uracil - analogs & derivatives, Uracil - metabolism, Viral Proteins - metabolism