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Epidemiological data on CD infection (CDI) in Latin American are scarce. CDI prevalence and strains characterization were prospectively evaluated in 5 Brazilian hospitals from different regions. Prevalence rates of CDI were 15%, ranging from 0 to 37%. ST42 was the most common Sequence Type and hypervirulent strains were not identified.
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•CDI prevalence and strains characterization were prospectively evaluated in 5 Brazilian hospitals from different regions.•CDI rates in Brazil were 15%, ranging from 0 to 37%.•High diversity of strains, with novel STs were found.•ST42 was the most common Sequence Type and classical hypervirulent strains were not identified.