Details

Autor(en) / Beteiligte

• Liu, Ran
• Yu, Dingchen
• Deng, Zixin
• Liu, Tiangang

Titel

Harnessing in vitro platforms for natural product research: in vitro driven rational engineering and mining (iDREAM)

Ist Teil von

• Current opinion in biotechnology, 2021-06, Vol.69, p.1-9

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •A research strategy for overproduction and discovery of natural products named iDREAM is proposed.•Attempts to harness in vitro platforms for target compound overproduction are reviewed.•Various novel natural products were rationally mined using in vitro platforms.•Technical challenges of in vitro natural product biosynthesis and solutions to date are summarized. Well-known issues amid in vivo research of natural product discovery and overproduction, such as unculturable or unmanipulable microorganisms, labor-intensive experimental cycles, and hidden rate-limiting steps, have hampered relevant investigations. To overcome these long-standing challenges, many researchers are turning toward in vitro platforms, which bypass the complicated cellular machinery and simplify the study of natural products. Here, we summarize the in vitro driven rational engineering and mining (iDREAM) strategy, which harnesses the flexibility and controllability of in vitro systems to rationally overproduce commodity chemicals and efficiently mine novel compounds. The iDREAM strategy promises to make further significant contributions toward both fundamental advances and industrial practices.