Details

Autor(en) / Beteiligte

• Huang, X.-F.
• Chen, P.-T.
• Lin, Y.-L.
• Lee, M.-S.
• Chang, K.-F.
• Liao, K.-W.
• Sheu, G.-T.
• Hsieh, M.-C.
• Tsai, N.-M.

Titel

Enhanced anticancer activity and endocytic mechanisms by polymeric nanocarriers of n-butylidenephthalide in leukemia cells

Ist Teil von

• Clinical & translational oncology, 2021-06, Vol.23 (6), p.1142-1151

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2021

Quelle

SpringerLink

Beschreibungen/Notizen

• Purpose The purpose of this study was to investigate the antitumor mechanisms of n -butylidenephthalide (BP) and to further examine the delivery efficacy of polycationic liposome containing PEI and polyethylene glycol complex (LPPC)-encapsulated BP in leukemia cells. Methods MTS, flow cytometric and TUNEL assays were performed to assess cell viability and apoptosis. BP and BP/LPPC complex delivery efficiency was analyzed by full-wavelength fluorescent scanner and fluorescence microscope. The expressions of cell cycle- and apoptosis-related proteins were conducted by Western blotting. Results The results showed that BP inhibited leukemia cell growth by inducing cell cycle arrest and cell apoptosis. LPPC-encapsulated BP rapidly induced endocytic pathway activation, resulting in the internalization of BP into leukemia cells, causing cell apoptosis within 1 h. Conclusions LPPC encapsulation enhanced the cytotoxic activity of BP and did not influence the effects of BP induction that suggested LPPC-encapsulated BP might be developed as anti-leukemia drugs in future.