Details

Autor(en) / Beteiligte

• da Silva, Natália Prado
• Carmo Rapozo Lavinas Pereira, Eloá do
• Duarte, Lucas Mattos
• de Oliveira Freitas, Jhamine Caroline
• de Almeida, Camila Guimarães
• da Silva, Thiago Pereira
• Melo, Rossana C.N.
• Morais Apolônio, Ana Carolina
• de Oliveira, Marcone Augusto Leal
• de Mello Brandão, Humberto
• Pittella, Frederico
• Fabri, Rodrigo Luiz
• Tavares, Guilherme Diniz
• de Faria Pinto, Priscila

Titel

Improved anti-Cutibacterium acnes activity of tea tree oil-loaded chitosan-poly(ε-caprolactone) core-shell nanocapsules

Ist Teil von

• Colloids and surfaces, B, Biointerfaces, 2020-12, Vol.196, p.111371-111371, Article 111371

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •Tea tree oil-loaded core-shell nanocapsules for acne treatment were prepared.•Chitosan was used to coat thenanocapsules containing tea tree oil.•The MIC of tea tree oil-loaded nanocapsules against C. acnes decreased four times.•Tea tree oil-loaded nanocapsules provided a decrease in C. acnes cell viability. The purpose of this study was to develop tea tree oil (TTO)-loaded chitosan-poly(ε-caprolactone) core-shell nanocapsules (NC-TTO-Ch) aiming the topical acne treatment. TTO was analyzed by gas chromatography-mass spectrometry, and nanocapsules were characterized regarding mean particle size (Z-average), polydispersity index (PdI), zeta potential (ZP), pH, entrapment efficiency (EE), morphology by Atomic Force Microscopy (AFM), and anti-Cutibacterium acnes activity. The main constituents of TTO were terpinen-4-ol (37.11 %), γ-terpinene (16.32 %), α-terpinene (8.19 %), ρ-cimene (6.56 %), and α-terpineol (6.07 %). NC-TTO-Ch presented Z-average of 268.0 ± 3.8 nm and monodisperse size distribution (PdI < 0.3). After coating the nanocapsules with chitosan, we observed an inversion in ZP to a positive value (+31.0 ± 1.8 mV). This finding may indicate the presence of chitosan on the nanocapsules' surface, which was corroborated by the AFM images. In addition, NC-TTO-Ch showed a slightly acidic pH (∼5.0), compatible with topical application. The EE, based on Terpinen-4-ol concentration, was approximately 95 %. This data suggests the nanocapsules' ability to reduce the TTO volatilization. Furthermore, NC-TTO-Ch showed significant anti-C. acnes activity, with a 4× reduction in the minimum inhibitory concentration, compared to TTO and a decrease in C. acnes cell viability, with an increase in the percentage of dead cells (17 %) compared to growth control (6.6 %) and TTO (9.7 %). Therefore, chitosan-poly(ε-caprolactone) core-shell nanocapsules are a promising tool for TTO delivery, aiming at the activity against C. acnes for the topical acne treatment.