Archives of biochemistry and biophysics, 2020-11, Vol.694, p.108594-108594, Article 108594
2020
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- Autor(en) / Beteiligte
- Titel
- Osteocytes promote osteoclastogenesis via autophagy-mediated RANKL secretion under mechanical compressive force
- Ist Teil von
- Archives of biochemistry and biophysics, 2020-11, Vol.694, p.108594-108594, Article 108594
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Osteocytes sense extracellular mechanical stimuli and transduce them into biochemical signals to regulate bone remodeling. The function is also evidenced in orthodontic tooth movement. But the underlying mechanisms haven't been clarified. Autophagy is an evolutionarily conserved cellular catabolic process which affects cellular secretory capabilities. We hypothesized that mechanical force activated osteocyte autophagy through TFE3-related signaling and further promoted osteocyte-mediated osteoclastogenesis. In the present study, we demonstrated that osteocyte autophagy was activated under mechanical compressive force using murine orthodontic tooth movement model since the number of LC3B-positive osteocytes increased by 3-fold in the compression side. In addition, both in vitro mechanical compression and chemical autophagy agonist increased the secretion of RANKL in osteocytes by 3-fold and 4-fold respectively, which is a crucial cytokine for osteoclastogenesis. Lastly, conditioned medium collected from compressed osteocytes promoted the development of osteoclasts. These results suggest that osteocytes could promote osteoclastogenesis via autophagy-mediated RANKL secretion under mechanical compressive force. Our research might provide evidence for exploring methods to accelerate tooth movement in clinic. •Osteocyte autophagy was activated during orthodontic tooth movement.•Osteocytes responded to in vitro mechanical compression.•Activated autophagy increased the secretion of RANKL in osteocytes.•Osteocytes promoted the development of osteoclasts when autophagy was activated.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0003-9861eISSN: 1096-0384DOI: 10.1016/j.abb.2020.108594Titel-ID: cdi_proquest_miscellaneous_2446679248
- Format
- –
- Schlagworte
- Animals, Autophagy, Autophagy - physiology, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors - metabolism, Cell Line, Male, Mechanical compression, Mice, Mice, Inbred C57BL, Osteoclastogenesis, Osteocytes, Osteocytes - metabolism, Osteogenesis - physiology, RANK Ligand - metabolism, Signal Transduction - physiology, Stress, Mechanical, Tooth Movement Techniques