Details

Autor(en) / Beteiligte

• De Luca, Arnaud
• Küster, Alice
• Tea, Illa
• Darmaun, Dominique
• Rozé, Jean-Christophe
• Robins, Richard
• Hankard, Régis

Titel

Plasma amino acid pools in the umbilical cord artery show lower 15N natural isotope abundance relative to the maternal venous pools

Ist Teil von

• Isotopes in environmental and health studies, 2021-03, Vol.57 (1), p.3-10

Ort / Verlag

Amsterdam: Taylor & Francis

Erscheinungsjahr

2021

Quelle

Taylor & Francis

Beschreibungen/Notizen

• 15 N natural isotope abundance (NIA) is systematically higher in infants' hair than in that of their mothers at birth. This study aimed to investigate this difference in plasma pools. We compared 15 N NIA values for plasma amino acid (AA) pools (free + protein-bound) in the umbilical cord artery (UCA) and vein (UCV) and in the maternal vein (MV) at birth. This preliminary study included 7 mother-infant dyads. Whole plasma was treated (HCl) to hydrolyze protein. Following derivatization, AAs were separated using gas chromatography and compound-specific 15 N NIA values were measured on-line using an isotope ratio monitoring mass spectrometer. 15 N NIA plasma AA pools in the UCA and UCV were highly correlated to the MV, r 2  > 0.89 and r 2  > 0.88 (both P < 10 −4 ) respectively. The full model found a significant effect of sampling compartment (P = 0.02) and AA type (P < 0.0001) on 15 N NIA plasma AA values. 15 N NIA plasma AA was 0.74 ‰ higher (P = 0.01) in the MV than in the UCA. This study indicates that a decrease in 15 N NIA for plasma AA pools occurs in the fetal-placental unit. Trial registration: ClinicalTrials.gov identifier: NCT00607061 .