Details

Autor(en) / Beteiligte

• Chanyalew, Menberework
• Abebe, Markos
• Endale, Birtukan
• Girma, Selfu
• Tasew, Geremew
• van Zandbergen, Ger
• Ritter, Uwe
• Gadisa, Endalamaw
• Aseffa, Abraham
• Laskay, Tamás

Titel

Enhanced production of pro-inflammatory cytokines and chemokines in Ethiopian cutaneous leishmaniasis upon exposure to Leishmania aethiopica

Ist Teil von

• Cytokine (Philadelphia, Pa.), 2021-09, Vol.145, p.155289-155289, Article 155289

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• •Cutaneous leishmaniasis (CL) in Ethiopia is mainly caused byL. aethiopica.•Response of CL patients to L. aethiopica was assessed using whole blood-based assay.•CL patients’ Leukocyte produced high level of inflammatory cytokien to L. aethiopica. The clinical course and outcome of cutaneous leishmaniasis (CL) vary due to the infecting Leishmania species and host genetic makeup that result in different immune responses against the parasites. The host immune response to Leishmania aethiopica (L.aethiopica), the causative agent of CL in Ethiopia, is poorly understood. To contribute to the understanding of the protective immune response in CL due to L.aethiopica, we characterized the cytokine response to L. aethiopica in patients with the localized form of CL (LCL) and age-and sex-matched apparently healthy controls. By applying a whole blood based in vitro culture we found enhanced release of TNF, IL–6, MCP-1 or CCL2, IP-10 or CXCL10, MIP-1β or CCL4 and IL-8 or CXCL8- but not of IL–10CL patients in response to L. aethiopica compared to the controls. No difference was observed between LCL cases and controls in the secretion of these cytokines and chemokines in whole blood cultures treated with the TLR-ligands LPS, MALP-2 or polyI: C. The observed increased secretion of the pro-inflammatory cytokines/chemokines reflects an enhanced response against the parasites by LCL patients as compared to healthy controls rather than a generally enhanced ability of blood leukocytes from LCL patients to respond to microbial constituents. Our findings suggest that the enhanced production of pro-inflammatory cytokines/chemokines is associated with localized cutaneous leishmaniasis caused by L.aethiopica.