Naunyn-Schmiedeberg's archives of pharmacology, 2021-02, Vol.394 (2), p.261-277
2021
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- Autor(en) / Beteiligte
- Titel
- Melatonin alleviates sepsis-induced heart injury through activating the Nrf2 pathway and inhibiting the NLRP3 inflammasome
- Ist Teil von
- Naunyn-Schmiedeberg's archives of pharmacology, 2021-02, Vol.394 (2), p.261-277
- Ort / Verlag
- Berlin/Heidelberg: Springer Berlin Heidelberg
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Melatonin improved the outcome of septic cardiomyopathy by inhibiting NLRP3 priming induced by reactive oxygen species. To get insights into these events, we studied the melatonin/Nrf2 antioxidant pathways during sepsis in the heart of NLRP3-deficient mice. Sepsis was induced by cecal ligation and puncture and melatonin was given at a dose of 30 mg/kg. Nuclear turnover of Nrf2 and p-Ser40 Nrf2 and expression of ho-1 were enhanced in nlrp3 +/+ and nlrp3 −/− mice during sepsis. Sepsis caused higher mitochondria impairment, apoptotic and autophagic events in nlrp3 +/+ mice than in nlrp3 −/− animals. These findings were accompanied by greater levels of Parkin and PINK-1, and lower Mfn2/Drp-1 ratio in nlrp3 +/+ than in nlrp3 −/− mice during sepsis, supporting less mitophagy in the latter. Ultrastructural analysis of myocardial tissue further confirmed these observations. The activation of NLRP3 inflammasome accounted for most of the deleterious effects of sepsis, whereas the Nrf2-dependent antioxidative response activation in response to sepsis was unable to neutralize these events. In turn, melatonin further enhanced the Nrf2 response in both mice strains and reduced the NLRP3 inflammasome activation in nlrp3 +/+ mice, restoring myocardial homeostasis. The data support that the anti-inflammatory efficacy of melatonin against sepsis depends, at least in part, on Nrf2 activation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0028-1298eISSN: 1432-1912DOI: 10.1007/s00210-020-01972-5Titel-ID: cdi_proquest_miscellaneous_2443518498
- Format
- –
- Schlagworte
- Animals, Antioxidants, Apoptosis, Apoptosis - drug effects, Biomedical and Life Sciences, Biomedicine, Cardiomyopathy, Cardiotonic Agents - pharmacology, Cardiotonic Agents - therapeutic use, Cecum, Cell Respiration - drug effects, Female, Heart Injuries - drug therapy, Heart Injuries - etiology, Heart Injuries - genetics, Heart Injuries - metabolism, Homeostasis, Inflammasomes, Inflammasomes - antagonists & inhibitors, Inflammasomes - genetics, Inflammation, Melatonin, Melatonin - pharmacology, Melatonin - therapeutic use, Mice, Mice, Inbred C57BL, Mice, Knockout, Mitochondria, Mitochondria, Heart - drug effects, Mitochondria, Heart - metabolism, Myocardium - metabolism, Myocardium - ultrastructure, Neurosciences, NF-E2-Related Factor 2 - genetics, NF-E2-Related Factor 2 - metabolism, NLR Family, Pyrin Domain-Containing 3 Protein - antagonists & inhibitors, NLR Family, Pyrin Domain-Containing 3 Protein - genetics, Original Article, Oxidoreductases - metabolism, Parkin protein, Pharmacology/Toxicology, Reactive oxygen species, Sepsis, Sepsis - complications, Sepsis - drug therapy, Sepsis - genetics, Sepsis - metabolism, Tumor Suppressor Protein p53 - genetics