Details

Autor(en) / Beteiligte

• He, Huagang
• Liu, Renkang
• Ma, Pengtao
• Du, Haonan
• Zhang, Huanhuan
• Wu, Qiuhong
• Yang, Lijun
• Gong, Shuangjun
• Liu, Tianlei
• Huo, Naxin
• Gu, Yong Q.
• Zhu, Shanying

Titel

Characterization of Pm68, a new powdery mildew resistance gene on chromosome 2BS of Greek durum wheat TRI 1796

Ist Teil von

• Theoretical and applied genetics, 2021, Vol.134 (1), p.53-62

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2021

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Key message New powdery mildew resistance gene Pm68 was found in the terminal region of chromosome 2BS of Greek durum wheat TRI 1796. The co-segregated molecular markers could be used for MAS. Durum wheat ( Triticum turgidum L. var. durum Desf.) is not only an important cereal crop for pasta making, but also a genetic resource for common wheat improvement. In the present study, a Greek durum wheat TRI 1796 was found to confer high resistance to all 22 tested isolates of Blumeria graminis f. sp. tritici ( Bgt ). Inheritance study on the F 1 plants and the F 2 population derived from the cross TRI 1796/PI 584832 revealed that the resistance in TRI 1796 was controlled by a single dominant gene, herein designated Pm68 . Using the bulked segregant RNA-Seq (BSR-Seq) analysis combined with molecular analysis, Pm68 was mapped to the terminal part of the short arm of chromosome 2B and flanked by markers Xdw04 and Xdw12 / Xdw13 with genetic distances of 0.22 cM each. According to the reference genome of durum wheat cv. Svevo, the corresponding physical region spanned the Pm68 locus was about 1.78-Mb, in which a number of disease resistance-related genes were annotated. This study reports the new powdery mildew resistance gene Pm68 that would be a valuable resource for improvement of both common wheat and durum wheat. The co-segregated markers ( Xdw05 – Xdw11 ) developed here would be useful tools for marker-assisted selection (MAS) in breeding.