Details

Autor(en) / Beteiligte

• Cáceres, Lourdes
• Paz, Mariela L.
• Garcés, Mariana
• Calabró, Valeria
• Magnani, Natalia D.
• Martinefski, Manuela
• Martino Adami, Pamela V.
• Caltana, Laura
• Tasat, Deborah
• Morelli, Laura
• Tripodi, Valeria
• Valacchi, Giuseppe
• Alvarez, Silvia
• González Maglio, Daniel
• Marchini, Timoteo
• Evelson, Pablo

Titel

NADPH oxidase and mitochondria are relevant sources of superoxide anion in the oxinflammatory response of macrophages exposed to airborne particulate matter

Ist Teil von

• Ecotoxicology and environmental safety, 2020-12, Vol.205, p.111186-111186, Article 111186

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Exposure to ambient air particulate matter (PM) is associated with increased cardiorespiratory morbidity and mortality. In this context, alveolar macrophages exhibit proinflammatory and oxidative responses as a result of the clearance of particles, thus contributing to lung injury. However, the mechanisms linking these pathways are not completely clarified. Therefore, the oxinflammation phenomenon was studied in RAW 264.7 macrophages exposed to Residual Oil Fly Ash (ROFA), a PM surrogate rich in transition metals. While cell viability was not compromised under the experimental conditions, a proinflammatory phenotype was observed in cells incubated with ROFA 100 μg/mL, characterized by increased levels of TNF-α and NO production, together with PM uptake. This inflammatory response seems to precede alterations in redox metabolism, characterized by augmented levels of H2O2, diminished GSH/GSSG ratio, and increased SOD activity. This scenario resulted in increased oxidative damage to phospholipids. Moreover, alterations in mitochondrial respiration were observed following ROFA incubation, such as diminished coupling efficiency and spare respiratory capacity, together with augmented proton leak. These findings were accompanied by a decrease in mitochondrial membrane potential. Finally, NADPH oxidase (NOX) and mitochondria were identified as the main sources of superoxide anion (▪) in our model. These results indicate that PM exposure induces direct activation of macrophages, leading to inflammation and increased reactive oxygen species production through NOX and mitochondria, which impairs antioxidant defense and may cause mitochondrial dysfunction. [Display omitted] •An inflammatory response precedes oxidative stress in PM-exposed RAW 264.7 cells.•H2O2 and ▪ are the main reactive oxygen species driving redox imbalance•PM exposure induces mitochondrial dysfunction in macrophages.•NADPH oxidase and mitochondria are the main sources of ▪.