Details

Autor(en) / Beteiligte

• Lai, Fangfang
• Deng, Wei
• Fu, Chunhua
• Wu, Pengbo
• Cao, Mingwei
• Tan, Shiyun

Titel

Long non‐coding RNA SNHG6 increases JAK2 expression by targeting the miR‐181 family to promote colorectal cancer cell proliferation

Ist Teil von

• The journal of gene medicine, 2020-12, Vol.22 (12), p.e3262-n/a

Ort / Verlag

England: Wiley Periodicals Inc

Erscheinungsjahr

2020

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Background Long non‐coding RNA (lncRNA) small nucleolar RNA host gene 6 (SNHG6) exerts a regulatory role in cancer biology, although its detailed functions and mechanisms in colorectal cancer (CRC) still remain unclear. Methods A quantitative reverse transcriptase‐polymerase chain reaction was implemented to investigate the expression of SNHG6, miR‐181 family and Janus kinase 2 (JAK2) in CRC tissues and cell lines. The proliferation of CRC cells was detected by a cell counting kit‐8 assay, and the apoptosis of CRC cells was determined by flow cytometry analysis. The interaction of the miR‐181 family with SNHG6 or with the 3'‐untranslated region of JAK2 was validated by the luciferase reporter gene method. The effects of SNHG6 and the miR‐181 family on JAK2 expression were analyzed by western blotting. Results SNHG6 was significantly up‐regulated in CRC samples. The knockdown of SNHG6 reduced the proliferation of CRC cells and promoted the apoptosis, whereas the over‐expression of SNHG6 had the opposite effect. SNHG6 could bind with all the four members of the miR‐181 family, and expression in miR‐181 family members was significantly down‐regulated in CRC samples. SNHG6 expression was negatively correlated with the miR‐181 family member expression in CRC samples. Moreover, over‐expressed SNHG6 significantly counteracted the inhibitory effect of miR‐181 mimics on CRC cell proliferation, as well as the promoting effect on apoptosis. Furthermore, SNHG6 over‐expression and knockdown can promote and inhibit JAK2 expression, respectively, and miR‐181 family member function is opposite to that of SNHG6 by repressing JAK2. Conclusions SNHG6 can exert a cancer‐promoting effect in CRC by targeting miR‐181 family members and up‐regulating JAK2. In the present study, we demonstrate that long non‐coding RNA small nucleolar RNA host gene 6 (SNHG6) is highly expressed in colorectal cancer (CRC) tissues and cells, and its abnormal overexpression can reduce the expression levels of miR‐181 family members via a competitive endogenous RNA mechanism, in turn up‐regulating the expression of Janus kinase 2. Via this mechanism, SNHG6 promotes the proliferation but inhibits the apoptosis of CRC cells.