Details

Autor(en) / Beteiligte

• Nakamura, Mitsuhiro
• Matsuda, Kazuo
• Morikawa, Takashi
• Ishizuka, Kohsuke
• Inouye, Satoshi

Titel

Efficient conversion to Cypridina luciferin from Cypridina luciferyl sulfate, coupled with enzymatic sulfation of acetic acid

Ist Teil von

• Biochemical and biophysical research communications, 2020-08, Vol.529 (3), p.678-684

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• In Cypridina (Vargula) hilgendorfii, Cypridina luciferin is converted from Cypridina luciferyl sulfate by a sulfotransferase with adenosine 3′, 5′-diphosphate (PAP), and is used for the luminescence reaction of Cypridina luciferase. We found that the luminescence activity of crude extracts of C. hilgendorfii was significantly stimulated by the addition of acetic acid. This stimulation may be explained by an efficient supply of PAP from 3′-phosphoadenosine 5′-phosphosulfate (PAPS) catalyzed by a sulfotransferase. Thus, acetic acid acts as a sulfate acceptor from PAPS, followed by forming acetyl sulfate and PAP. The structure of acetyl sulfate was identified using mass spectrometry and it spontaneously decomposed to acetic acid and free sulfate ion in aqueous solutions. This enzymatic conversion from Cypridina luciferyl sulfate to Cypridina luciferin could be coupled with acetic acid and PAPS by a sulfotransferase. [Display omitted] •Cypridina luciferyl sulfate is converted to Cypridina luciferin by a sulfotransferase.•Acetic acid stimulates luminescence activity in crude extracts of Cypridina specimens.•Acetic acid is sulfated with PAPS to produce acetyl sulfate in crude extracts.•Production of acetyl sulfate and PAP stimulates Cypridina luciferin formation.•Unstable acetyl sulfate is decomposed to acetic acid and SO42− in aqueous solutions.