Details

Autor(en) / Beteiligte

• Huang, QiuYuan
• Hiyama, Masatoshi
• Kabe, Taizo
• Kimura, Satoshi
• Iwata, Tadahisa

Titel

Enzymatic Self-Biodegradation of Poly(l‑lactic acid) Films by Embedded Heat-Treated and Immobilized Proteinase K

Ist Teil von

• Biomacromolecules, 2020-08, Vol.21 (8), p.3301-3307

Ort / Verlag

American Chemical Society

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Non-biodegradable microplastics have become a global problem. We propose a new enzyme-embedded biodegradable plastic that can be self-biodegraded anytime and anywhere. Proteinase K from Tritirachium album was embedded in poly­(l-lactic acid) (PLLA). The PLLA solution-cast film with embedded proteinase K showed weight loss of 78% after 96 h incubation. In addition, PLLA extruded films embedding immobilized proteinase K encapsulated in polyacrylamide were produced at 200 °C and embedded-enzyme degradation was monitored. Immobilized proteinase K embedded in the extruded film maintained its degradation activity and degraded the PLLA film from inside to make small holes and cavities, suggesting that immobilization is a powerful technique to prepare thermoforms with embedded enzymes. The rate of embedded-enzyme degradation was accelerated by dividing the film into smaller pieces, which can be regarded as a model experiment for biodegradation of microplastics. Various biodegradable plastics with specific embedded enzymes will contribute to solve global environmental problems.