Details

Autor(en) / Beteiligte

• Rao, Chun-Bao
• Luo, Dong
• Lin, Zi-Tian
• Xie, Ming-Yu
• Hu, Yuan
• Peng, Qi
• Jiang, Hua
• Zhang, Zhen-Hong
• Lu, Xiao-Mei

Titel

Expression of long non-coding RNA linc00467 in childhood acute myeloid leukemia and its role in drug resistance

Ist Teil von

• Zhongguo dang dai er ke za zhi, 2020-07, Vol.22 (7), p.734

Ort / Verlag

China

Erscheinungsjahr

2020

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• To study the expression and function of long non-coding RNA linc00467 in childhood acute myeloid leukemia (AML). Bone marrow samples were collected from 5 children with AML who were diagnosed from May 2016 to June 2018. Normal bone marrow samples based on bone marrow examination were collected from 3 children as controls. Quantitative real-time PCR was used to measure the expression of linc00467 in the two groups. A lentivirus system was used to achieve overexpression of linc00467 in AML cells (HL-60) (linc00467 overexpression group), and empty vector expressing green fluorescent protein (GFP) was transfected into AML cells to establish a GFP control group. A lentivirus system was used to insert an interfering sequence into AML cells (sh-linc00467 interfering group), and a random sequence was inserted to establish an sh-NC control group. Cell proliferation and resistance to doxorubicin were observed for all groups. Compared with the normal control group, the children with AML had a significant increase in lin