Details

Autor(en) / Beteiligte

• Joo, Min-Soo
• Choi, Kwang-Min
• Cho, Dong-Hee
• Choi, Hye-Sung
• Min, Eun Young
• Han, Hyun-Ja
• Cho, Mi Young
• Bae, Jin-Sol
• Park, Chan-Il

Titel

The molecular characterization, expression analysis and antimicrobial activity of theromacin from Asian polychaeta (Perinereis linea)

Ist Teil von

• Developmental and comparative immunology, 2020-11, Vol.112, p.103773-103773, Article 103773

Ort / Verlag

United States: Elsevier Ltd

Erscheinungsjahr

2020

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Antimicrobial peptides (AMPs) are molecular factors in innate immunity and are believed to play a key role in invertebrate host defence. We identified theromacin (TM) from an Asian polychaeta, Perinereis linea, using de novo RNA-seq analysis. TM, a typical AMP of invertebrates, is a cysteine-rich AMP with five disulfide bonds consisting of ten cysteine residues. In gene expression analysis, TM genes were constantly upregulated after lipopolysaccharide (LPS) stimulation. In contrast, after peptidoglycan (PGN) stimulation, it was upregulated initially and downregulated after 12 h. We synthesized a peptide based on the macin AMP in the TM amino acid sequence. The synthetic peptide showed antibacterial activity against some Gram-positive and Gram-negative bacteria. Therefore, the AMPs of P. linea might have broad roles in host defence and exhibit different degrees of activity. •The PlTM conserved macin domain was important to antimicrobial activities.•The PlTM shows high expression after stimulation with LPS and PGN.•The sPlTM exhibited a broad antibacterial activity against various bacteria.•The PlTM plays an important role in the innate immunity of Perinereis linea.