Details

Autor(en) / Beteiligte

• Dietrich, Jens Erik
• Jauckus, Julia
• Hoffmann, Sabrina
• Liebenthron, Jana
• Capp, Edison
• Strowitzki, Thomas
• Germeyer, Ariane

Titel

In vitro maturation of immature oocytes from ovarian tissue prior to shipment to a cryobank

Ist Teil von

• Archives of gynecology and obstetrics, 2020-10, Vol.302 (4), p.1019-1024

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Purpose Female fertility preservation prior to gonadotoxic therapies can be achieved by the cryopreservation of ovarian cortical tissue. Immature oocytes may be recovered during the preparation, matured in vitro and lead to live births, thereby providing an additional option for fertility preservation. The purpose of this study was to test the feasibility of this approach in a setting with unilateral biopsy of a small piece of ovarian tissue and minimal tissue preparation prior to shipment to an external cryobank. Methods A prospective observational clinical study in an academic center was performed from January 2018 through December 2019. Ovarian tissue was obtained laparoscopically. Immature oocytes were recovered by minimal preparation of the tissue before shipment to an external cryobank for cryopreservation. In vitro maturation was performed on recovered immature oocytes. Results Twelve patients were enrolled. Immature oocytes could be recovered for all. The maturation rate was 38.9% ( n  = 14/36). Metaphase II (MII) were either directly used for intracytoplasmic sperm injection (ICSI) with a fertilization rate of 66.6% ( n  = 4/6) or vitrified ( n  = 8). PNs were cryopreserved ( n  = 4). Vitrified MII were warmed with a post-warming vitality rate of 75.0% ( n  = 3/4) and used for ICSI with a fertilization rate of 33.3% ( n  = 1/3). Conclusions Immature oocytes can be successfully retrieved from ovarian tissue through minimal tissue preparation prior to shipment to a cryobank, matured in vitro, fertilized and cryopreserved for potential future fertility treatments. The total number of oocytes available for fertility preservation can be increased even without controlled ovarian stimulation in a situation where only ovarian biopsy for cryopreservation is performed. Trial registration German Clinical Trials Register (DRKS), DRKS00013170. Registered 11 December 2017, https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00013170 .