Details

Autor(en) / Beteiligte

• Vidal, Livia M. R.
• Venas, Tainá M.
• Gonçalves, Aline R. P.
• Mattsson, Hannah K.
• Silva, Raphael V. P.
• Nóbrega, Maria S.
• Azevedo, Gustavo P. R.
• Garcia, Gizele D.
• Tschoeke, Diogo A.
• Vieira, Verônica V.
• Thompson, Fabiano L.
• Thompson, Cristiane C.

Titel

Rapid screening of marine bacterial symbionts using MALDI-TOF MS

Ist Teil von

• Archives of microbiology, 2020-10, Vol.202 (8), p.2329-2336

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a rapid, cost-effective and high-throughput method for bacteria characterization. However, most previous studies focused on clinical isolates. In this study, we evaluated the use of MALDI-TOF MS as a rapid screening tool for marine bacterial symbionts. A set of 255 isolates from different marine sources (corals, sponge, fish and seawater) was analyzed using cell lysates to obtain a rapid grouping. Cluster analysis of mass spectra and 16S rRNA showed 18 groups, including Vibrio, Bacillus, Pseudovibrio, Alteromonas and Ruegeria . MALDI-TOF distance similarity scores ≥ 60% and ≥ 70% correspond to ≥ 98.7% 16S rRNA gene sequence similarity and ≥ 95% pyrH gene sequence similarity, respectively. MALDI-TOF MS is a useful tool for Vibrio species groups’ identification.