Oncogene, 2020-06, Vol.39 (26), p.4956-4969
2020
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Details
- Autor(en) / Beteiligte
- Titel
- HDAC8 promotes the dissemination of breast cancer cells via AKT/GSK-3β/Snail signals
- Ist Teil von
- Oncogene, 2020-06, Vol.39 (26), p.4956-4969
- Ort / Verlag
- London: Nature Publishing Group UK
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The mechanistic action of histone deacetylase 8 (HDAC8) in cancer motility, including epithelial-mesenchymal transition (EMT), remains largely undefined. We found that the expression of HDAC8 was upregulated in breast cancer (BC) cells and tissues as compared to the controls. Further, BC tissues had the highest values of HDAC8 expression among 31 kinds of cancers. Cellular study indicated that HDAC8 can positively regulate the dissemination and EMT of BC cells. It increased the protein stability of Snail, an important regulator of EMT, by phosphorylation of its motif 2 in serine-rich regions. There are 21 factors that have been reported to regulate the protein stability of Snail. Among them, HDAC8 can decrease the expression of GSK-3β through increasing its Ser9-phosphorylation. Mass spectrum analysis indicated that HDAC8 interact with AKT1 to decrease its acetylation while increase its phosphorylation, which further increased Ser9-phosphorylation of GSK-3β. The C-terminal of AKT1 was responsible for the interaction between HDAC8 and AKT1. Further, Lys426 was the key residue for HDAC8-regulated deacetylation of AKT1. Moreover, HDAC8/Snail axis acted as adverse prognosis factors for in vivo progression and overall survival (OS) rate of BC patients. Collectively, we found that HDAC8 can trigger the dissemination of BC cells via AKT/GSK-3β/Snail signals, which imposed that inhibition of HDAC8 is a potential approach for BC treatment.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0950-9232eISSN: 1476-5594DOI: 10.1038/s41388-020-1337-xTitel-ID: cdi_proquest_miscellaneous_2410354095
- Format
- –
- Schlagworte
- 13/1, 13/2, 13/21, 13/95, 14/1, 14/19, 42/109, 42/89, 631/67/395, 631/80/83, Acetylation, AKT protein, AKT1 protein, Animals, Apoptosis, Breast cancer, Breast Neoplasms - genetics, Breast Neoplasms - metabolism, Breast Neoplasms - therapy, Cell Biology, Cell Line, Tumor, Cell Movement - genetics, Deacetylation, Female, Gene Expression Regulation, Neoplastic, Glycogen Synthase Kinase 3 beta - genetics, Glycogen Synthase Kinase 3 beta - metabolism, Histone deacetylase, Histone Deacetylases - genetics, Histone Deacetylases - metabolism, Human Genetics, Humans, Internal Medicine, Kaplan-Meier Estimate, Kinases, Medical prognosis, Medicine, Medicine & Public Health, Mesenchyme, Mice, Nude, Oncology, Phosphorylation, Proto-Oncogene Proteins c-akt - genetics, Proto-Oncogene Proteins c-akt - metabolism, Repressor Proteins - genetics, Repressor Proteins - metabolism, Serine, Signal Transduction - genetics, Snail Family Transcription Factors - genetics, Snail Family Transcription Factors - metabolism, Xenograft Model Antitumor Assays - methods