Details

Autor(en) / Beteiligte

• Wu, Zhongzhen
• Cui, Yang
• Ma, Jun
• Qu, Mengqiu
• Lin, Jintian

Titel

Analyses of chemosensory genes provide insight into the evolution of behavioral differences to phytochemicals in Bactrocera species

Ist Teil von

• Molecular phylogenetics and evolution, 2020-10, Vol.151, p.106858-106858, Article 106858

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• [Display omitted] •Chemosensory genes were identified from antennae of five Bactrocera species.•Chemosensory genes were found to be under less purifying selection.•Differential expression of chemosensory genes was shown among ME- and RK-responders.•Divergence in chemosensory genes are likely responsible for ME- and RK-perception. Insect olfactory systems have evolved to recognize phytochemicals and respond to olfactory-triggered cues that vary depending on needs. Several Bactrocera species are attracted explicitly to particular phytochemicals, including methyl eugenol (ME) and cue lure/raspberry ketone (CL/RK). The attraction of Bactrocerans to ME/RK is primarily driven by olfaction. Therefore, the divergent behavioral phenotypes are due to the differences in genes expressed in antennae. High quality transcriptomes were generated with mRNA from dissected antennae, to analyze the differences in olfaction-related genes of three ME-responders B. dorsalis, B. papayae and B. correcta and two RK-responders B. cucurbitae and B. tau. Many distinct quantitative and qualitative differences were identified in their respective chemosensory repertoires. Tissue- and sex-specific expression analyses identified antennae-predominant and sex-biased chemosensory genes. Sequence comparison revealed variations among family members of odorant-binding proteins and odorant-receptors between ME-responders and RK-responders. Differences in composition, expression levels, and sequence of proteins encoded by olfactory-related genes were identified between ME-responders and RK-responders. Some of the differences might contribute to the divergence in response to plant-derived odorants. Taken together, our results provide insights into the evolution of an olfactory system at molecular level in Bactrocera ME- and CL/RK-responding species.