Details

Autor(en) / Beteiligte

• Liu, Ling
• Li, Sainan
• Sun, Runze
• Qin, Xiangjing
• Ju, Jianhua
• Zhang, Changsheng
• Duan, Yanwen
• Huang, Yong

Titel

Activation and Characterization of Bohemamine Biosynthetic Gene Cluster from Streptomyces sp. CB02009

Ist Teil von

• Organic letters, 2020-06, Vol.22 (12), p.4614-4619

Ort / Verlag

United States

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Bohemamines (BHMs) are bacterial alkaloids containing a pyrrolizidine core with two unusual methyl groups. Herein we report the activation of BHMs biosynthesis using a ribosome engineering approach. Characterization of the gene cluster reveals that nonribosomal peptide synthetase BhmJ and Baeyer-Villiger monooxygenase BhmK are responsible for the formation of the pyrrolizidine core, which is further methylated on C-7 by methyltransferase BhmG. The 9-methyl group of BHMs is instead originated from a nonproteinogenic amino acid (2 ,5 )-5-methylproline.