Details

Autor(en) / Beteiligte

• Hirashima, Shingo
• Ohta, Keisuke
• Kanazawa, Tomonoshin
• Togo, Akinobu
• Tsuneyoshi, Risa
• Miyazono, Yoshihiro
• Kusukawa, Jingo
• Nakamura, Kei-ichiro

Titel

Correlative imaging of collagen fibers and fibroblasts using CLEM optimized for picrosirius red staining and FIB/SEM tomography

Ist Teil von

• Microscopy, 2020-10, Vol.69 (5), p.324-329

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2020

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Abstract Conventional imaging for three-dimensional (3D) ultra-architectural analysis of collagen fibers and fibroblasts is time-consuming and requires numerous ultrathin sections to search the target area. Currently, no method allows 3D ultra-architectural analysis of predetermined areas including spatial relationships between collagen fibers and fibroblasts in vitro. Herein, we developed a new method for in vitro analysis of the 3D ultrastructure of fibroblasts and collagen fibers using CLEM optimized for picrosirius red staining and FIB/SEM tomography. Collagen fibers were observed between, rather than on top of, stacked cells. This method offers the advantage of mesoscopic and ultrastructural analysis, thus minimizing bias and ensuring accurate observation.