Nature biotechnology, 2020-05, Vol.38 (5), p.582-585
2020
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Details
- Autor(en) / Beteiligte
- Titel
- Prime genome editing in rice and wheat
- Ist Teil von
- Nature biotechnology, 2020-05, Vol.38 (5), p.582-585
- Ort / Verlag
- New York: Nature Publishing Group US
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Prime editors, which are CRISPR–Cas9 nickase (H840A)–reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%. Prime editors are optimized for use in plants.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1087-0156eISSN: 1546-1696DOI: 10.1038/s41587-020-0455-xTitel-ID: cdi_proquest_miscellaneous_2401823149
- Format
- –
- Schlagworte
- 631/1647, 631/45, 631/61/447, Agriculture, Binding sites, Bioinformatics, Biomedical and Life Sciences, Biomedical Engineering/Biotechnology, Biomedicine, Biotechnology, Brief Communication, Cells, Codon, CRISPR, CRISPR-Cas Systems, Deoxyribonuclease I - metabolism, Deoxyribonucleic acid, DNA, DNA damage, DNA polymerases, DNA repair, Editing, Efficiency, Gene Editing - methods, Genome editing, Genome, Plant, Genomes, Genomics, Life Sciences, Mammalian cells, Mutation, Optimization, Oryza - genetics, Oryza - growth & development, Protoplasts, Rice, RNA-directed DNA polymerase, Triticum - genetics, Triticum - growth & development, Wheat