Details

Autor(en) / Beteiligte

• de Almeida Barros, Vanessa
• Fontes, Patrícia Pereira
• Barcelos de Souza, Gilza
• Gonçalves, Amanda Bonoto
• de Carvalho, Kenia
• Rincão, Michelle Pires
• de Oliveira Negrão Lopes, Ivani
• Dal-Bianco Lamas Costa, Maximiller
• Alves, Murilo Siqueira
• Marcelino-Guimarães, Francismar Corrêa
• Fietto, Luciano Gomes

Titel

Phakopsora pachyrhizi triggers the jasmonate signaling pathway during compatible interaction in soybean and GmbZIP89 plays a role of major component in the pathway

Ist Teil von

• Plant physiology and biochemistry, 2020-06, Vol.151, p.526-534

Ort / Verlag

France: Elsevier Masson SAS

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• The biotrophic fungus Phakopsora pachyrhizi is currently the major pathogen affecting soybean production worldwide. It has already been suggested for the non-host interaction between P. pachyrhizi and Arabidopsis thaliana that the fungus in early infection induces jasmonic acid (JA) pathway to the detriment of the salicylic acid (SA) pathway as a mechanism to the establishment of infection. In this study, we verified that this mechanism might also be occurring during the compatible interaction in soybean (Glycine max L. Merril). It was demonstrated that P. pachyrhizi triggers a JA pathway during the early and late stages of infection in a susceptible soybean cultivar. The expression of the GmbZIP89 was induced in a biphasic profile, similarly to other JA responsive genes, which indicates a new marker gene for this signaling pathway. Additionally, plants silenced for GmbZIP89 (iGmZIP89) by the virus-induced gene silencing (VIGS) approach present lower severity of infection and higher expression of pathogenesis related protein 1 (PR1). The lower disease severity showed that the iGmbZIP89 plants became more resistant to infection. These data corroborate the hypothesis that the GmbZIP89 may be a resistance negative regulator. In conclusion, we demonstrated that P. pachyrhizi mimics a necrotrophic fungus and activates the JA/ET pathway in soybean. It is possible to suppose that its direct penetration on epidermal cells or fungal effectors may modulate the expression of target genes aiming the activation of the JA pathway and inhibition of SA defense. •Phakopsora pachyrhizi triggers a JA pathway in early and late infection in soybean.•P. pachyrhizi mimics the attack of a necrotrophic pathogen in soybean.•GmbZIP89 is a new marker gene for the JA signaling pathway.•GmbZIP89 silenced plants showed higher expression of PR1.•GmBZIP89 negatively regulates resistance against Phakopsora pachyrhizi in soybean.