Details

Autor(en) / Beteiligte

• Hu, Kaidi
• Wang, Xingjie
• Zhu, Jiawen
• Liu, Aiping
• Ao, Xiaolin
• He, Li
• Chen, Shujuan
• Zhou, Kang
• Yang, Yong
• Zou, Likou
• Liu, Shuliang

Titel

Characterization of carbaryl-degrading strain Bacillus licheniformis B-1 and its hydrolase identification

Ist Teil von

• Biodegradation (Dordrecht), 2020-04, Vol.31 (1-2), p.139-152

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Pesticides introduced inadvertently or deliberately into environment by anthropogenic activity have caused growing global public concern, therefore the search of approaches for elimination of such xenobiotics should be encouraged. A cypermethrin-degrading bacterial strain Bacillus licheniformis B-1 was found to efficiently degrade carbaryl in LB medium at concentrations of 50–300 mg L −1 within 48 h, during which temperature and pH played important roles as reflected by increase in pollutant depletion. A stimulatory effect of Fe 3+ and Mn 2+ on microbial growth was observed, whereas Cu 2+ caused inhibition of degradation. Results showed that 1-naphthol was a major transformation product of carbaryl which was further metabolised. An approximately 29 kDa carbaryl-degrading enzyme was purified from B-1 with 15.93-fold purification and an overall yield of 6.02% was achieved using ammonium sulphate precipitation, DEAE-Sepharose CL-6B anion-exchange chromatography and Sephadex G-100 gel filtration. The enzyme was identified through nano reversed-phase liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry as a phosphodiesterase (PDE). This is the first report on the characterization of carbaryl-degrading by Bacillus spp. and the role of a PDE in carbaryl-detoxifying. Also, strain B-1 showed versatile in carbosulfan, isoprocarb and chlorpyrifos degradation, demonstrating as ideal candidate for environment bioremediation.