Details

Autor(en) / Beteiligte

• Zhao, Juanjuan
• Yuan, Quan
• Wang, Haiyan
• Liu, Wei
• Liao, Xuejiao
• Su, Yingying
• Wang, Xin
• Yuan, Jing
• Li, Tingdong
• Li, Jinxiu
• Qian, Shen
• Hong, Congming
• Wang, Fuxiang
• Liu, Yingxia
• Wang, Zhaoqin
• He, Qing
• Li, Zhiyong
• He, Bin
• Zhang, Tianying
• Fu, Yang
• Ge, Shengxiang
• Liu, Lei
• Zhang, Jun
• Xia, Ningshao
• Zhang, Zheng

Titel

Antibody Responses to SARS-CoV-2 in Patients With Novel Coronavirus Disease 2019

Ist Teil von

• Clinical infectious diseases, 2020-11, Vol.71 (16), p.2027-2034

Ort / Verlag

US: Oxford University Press

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Abstract Background The novel coronavirus SARS-CoV-2 is a newly emerging virus. The antibody response in infected patients remains largely unknown, and the clinical value of antibody testing has not been fully demonstrated. Methods 173 patients with SARS-CoV-2 infection were enrolled. Their serial plasma samples (n = 535) collected during hospitalization were tested for total antibodies (Ab), IgM, and IgG against SARS-CoV-2. The dynamics of antibodies with disease progress were analyzed. Results Among 173 patients, the seroconversion rates for Ab, IgM, and IgG were 93.1%, 82.7%, and 64.7%, respectively. The reason for the negative antibody findings in 12 patients might be due to the lack of blood samples at the later stage of illness. The median seroconversion times for Ab, IgM, and then IgG were days 11, 12, and 4, respectively. The presence of antibodies was <40% among patients within 1 week of onset, and rapidly increased to 100.0% (Ab), 94.3% (IgM), and 79.8% (IgG) by day 15 after onset. In contrast, RNA detectability decreased from 66.7% (58/87) in samples collected before day 7 to 45.5% (25/55) during days 15–39. Combining RNA and antibody detection significantly improved the sensitivity of pathogenic diagnosis for COVID-19 (P < .001), even in the early phase of 1 week from onset (P = .007). Moreover, a higher titer of Ab was independently associated with a worse clinical classification (P = .006). Conclusions Antibody detection offers vital clinical information during the course of SARS-CoV-2 infection. The findings provide strong empirical support for the routine application of serological testing in the diagnosis and management of COVID-19 patients. Combining RNA and antibody detection significantly improved the sensitivity of pathogenic diagnosis for coronavirus disease 2019 COVID-19 in the early phase of infection. A higher titer of total antibodies was independently associated with a worse clinical classification.