Details

Autor(en) / Beteiligte

• Xie, S Y
• Wu, Q Q
• Liu, C
• Deng, W
• Tang, Q Z

Titel

The effect and mechanism of heat shock protein 47 on streptozotocin-induced diabetic cardiomyopathy

Ist Teil von

• Zhong hua yi xue za zhi, 2020-02, Vol.100 (6), p.430-436

Ort / Verlag

China

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the effect and specific mechanism of heat shock protein 47 (HSP47) on streptozotocin (STZ)-induced diabetic cardiomyopathy (DCM). A mouse model of type 1 diabetic cardiomyopathy was established by intraperitoneal injection of STZ. After 8 weeks of successful modeling, HSP47 was overexpressed by tail vein injection, and the heart of the mouse was harvested after 6 weeks. Hematoxylin-eosin (HE) staining and Sirius red (PSR) staining were used to detect the cross-sectional area of myocardial cells and myocardial fibrosis, respectively. Immunofluorescence staining with α-smooth muscle actin (α-SMA) and collagen Ⅰ was used to detect the degree of fibrosis activation. The expression level of fibrosis-related proteins was determined by Western blot. The expression level of HSP47 in the myocardium of the diabetic group up-regulated (2.014±0.264 vs 1.004±0.064, 0.001). The area of myocardial cells in the diabetic group was increased compared with the control group [(235.3±20.7) μm(2) vs (172.8±13.6) μm(