Details

Autor(en) / Beteiligte

• Fan, Linyuan
• Xu, Haoying
• Li, Di
• Li, Hongling
• Lu, Dan

Titel

A novel long noncoding RNA, AC092834.1, regulates the adipogenic differentiation of human adipose-derived mesenchymal stem cells via the DKK1/Wnt/β-catenin signaling pathway

Ist Teil von

• Biochemical and biophysical research communications, 2020-05, Vol.525 (3), p.747-754

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Long noncoding RNAs (lncRNAs) have been implicated in a range of developmental processes and diseases, but the roles and mechanisms by which they act in adipogenic differentiation and adipose tissue biology are still unknown. By comparing the different expression patterns of lncRNAs before and after the adipocyte differentiation of human adipose-derived mesenchymal stem cells (hADSCs), we characterized a novel lncRNA, AC092834.1, which is significantly increased in preadipocytes. By gain- and loss-of-function experiments, we demonstrated that lncRNA AC092834.1 potentiated adipogenic differentiation through directly increasing the level of expression of DKK1, which competitively binds with LRP5 to inhibit the Wnt-β-catenin pathway and reduce the inhibition of adipogenesis by Wnt signaling. This finding provides novel mechanistic insights into a critical role for lncRNA AC092834.1 as a regulator of adipogenic differentiation, which expands our knowledge about the molecular mechanisms of obesity and other adipogenic differentiation-related disorders. •The novel lncRNA, AC092834.1, is upregulated in hADSCs adipogenesis.•LncRNA AC092834.1 could positive regulate adipogenic differentiation of hADSCs.•LncRNA AC092834.1 induce DKK1 expression and antagonize Wnt/β-catenin pathway.