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Wearable sweat sensors demonstrate outstanding performance in non-invasive, real-time monitoring of vital biomarkers in sweat, which offer an opportunity for individuals to achieve dynamic monitoring their own physiology in molecular-level. As a key step in sweat analysis that impact the accuracy of results, frequently-used sweat sampling methods are introduced in this review, and the emphasis is sweat sampling in wearable sensors including absorbent materials, superhydrophobic/superhydrophilic surface, sweat guidance and epidermal microfluidic systems. In the end, we also propose the remaining challenges in the practical, large-scale application of wearable sweat sensors and provide personal prospects on the future development.
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•This paper summarized frequently-used sampling methods in emerging wearable sweat sensors.•The basic procedure, advantages and disadvantages of each sampling method have been introduced in details.•Non-invasive wearable sweat devices are promising for better personalized and predictive healthcare after overcoming several remaining challenges.