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Quantitative Bioimage Analysis of Passaging Effect on the Migratory Behavior of Human Mesenchymal Stem Cells During Spheroid Formation
Cytometry. Part A, 2020-04, Vol.97 (4), p.394-406
Jiang, Ching‐Fen
Hsu, Shan‐hui
Sun, Yu‐Man
Tsai, Ming‐Hong
2020
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Jiang, Ching‐Fen
Hsu, Shan‐hui
Sun, Yu‐Man
Tsai, Ming‐Hong
Titel
Quantitative Bioimage Analysis of Passaging Effect on the Migratory Behavior of Human Mesenchymal Stem Cells During Spheroid Formation
Ist Teil von
Cytometry. Part A, 2020-04, Vol.97 (4), p.394-406
Ort / Verlag
Hoboken, USA: John Wiley & Sons, Inc
Erscheinungsjahr
2020
Quelle
Wiley Online Library Journals Frontfile Complete
Beschreibungen/Notizen
The quality of stem cells obtained through serial subcultivation is the pivotal factor determining the therapeutic effectiveness of regenerative medicine. However, an effective quality monitoring system for cell culture is yet to be established. Detailed parameter studies of the migratory behavior of stem cells at different passages may provide insight into the deterioration of stemness. Thus, this study aimed to evaluate the feasibility of quantitative bioimage analysis for monitoring stem cell quality during in vitro culture and to explore the passaging effects on stem cell migration. An image‐based analytical tool using cell tracking, cytometric analyses, and gating with time‐lapse microscopy was developed to characterize the migratory behavior of human mesenchymal stem cells (hMSCs) isolated from human adipose tissue (hADAS) and placenta (hPDMC) cultured on chitosan membranes. Quantitative analysis was performed for the single cells and assembled spheroids selected from 15 videos of Passages 3, 5, and 11 for hADAS and those from 12 videos of Passages 7, 11, and 16 for hPDMC. These passages were selected to represent the young, matured, and degenerated stem cells, respectively. Migratory behavior varied with cell passages. The mobility of single hMSCs decreased at degenerated passages. In addition, enhancement of mobility, due to transformation from single cells to spheroids, occurred at each passage. The young hMSCs seemed more likely to move as single cells rather than as aggregates. Once matured, they tended to aggregate with strong 3D spheroid formability and increased mobility. However, the spheroid formability and mobility decreased at late passage. The increase in aggregation rate with passaging may be a compensatory mechanism to enhance the declining mobility of hMSCs through cell coordination. Our findings regarding the passaging effects on stem‐cell migratory behavior agree with biochemical reports, suggesting that the developed imaging method is capable of monitoring the cell‐culture quality effectively. © 2020 International Society for Advancement of Cytometry
Sprache
Englisch
Identifikatoren
ISSN: 1552-4922
eISSN: 1552-4930
DOI: 10.1002/cyto.a.23985
Titel-ID: cdi_proquest_miscellaneous_2369396810
Format
–
Schlagworte
Adipose tissue
,
Cell adhesion & migration
,
Cell culture
,
Cell migration
,
Chitosan
,
Cytometry
,
Formability
,
Gating
,
Mesenchymal stem cells
,
migratory behavior
,
Mobility
,
Monitoring
,
passaging effect
,
Placenta
,
Quantitative analysis
,
quantitative bioimage analysis
,
Regenerative medicine
,
Spheroids
,
stem cell
,
Stem cells
,
Tissue engineering
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