Details

Autor(en) / Beteiligte

• Almaz, Zuleyha
• Oztekin, Aykut
• Abul, Nurgul
• Gerni, Serpil
• Erel, Deniz
• Kocak, Seyma Mehtap
• Sengül, Mehmet Emin
• Ozdemir, Hasan

Titel

A new approach for affinity‐based purification of horseradish peroxidase

Ist Teil von

• Biotechnology and applied biochemistry, 2021-02, Vol.68 (1), p.102-113

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• We have developed efficient procedure for isolation of horseradish peroxidase (HRP) using aminobenzohydrazide‐based affinity chromatography. Sepharose 4B‐bounded aminobenzohydrazides are suitable for long‐term use and large‐scale purification. In this study, 26 aminobenzohydrazide derivatives were synthesized, characterized and defined as new HRP inhibitors. In addition, detailed inhibition effects of these molecules on HRP enzyme were investigated. Affinity matrix was formed by bonding aminobenzohydrazides, which exhibited inhibitory activity to sepharose‐4B‐l‐tyrosine. HRP was isolated from crude homogenate in single step and purification factors were recorded as 1,151‐fold (recovery of 8.5%) with 4‐amino 3‐bromo benzohydrazide and as 166.16‐fold (recovery of 16.67 %) with 3‐amino 4‐chloro benzohydrazide. Purification steps scheme of HRP