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Overexpression of global regulator LaeA increases secondary metabolite production in Monascus purpureus
Ist Teil von
Applied microbiology and biotechnology, 2020-04, Vol.104 (7), p.3049-3060
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2020
Link zum Volltext
Quelle
SpringerNature Journals
Beschreibungen/Notizen
Monascus
is a filamentous fungus that produces several secondary metabolites. Here, we investigated the effects of the global regulator
LaeA
on the synthesis of pigments and monacolin K in
Monascus purpureus
with spectrophotometer and HPLC methods. The
LaeA
gene was isolated from
M. purpureus
M1 to create an overexpression construct. An
LaeA
-overexpressing strain L3 was with 48.6% higher monacolin K production than the M1 strain. The L3 strain also produced higher
Monascus
pigments than the M1 strain. SEM showed that
LaeA
overexpression resulted in altered mycelial morphology. Compared with the M1 strain, the L3 strain expressed higher levels of monacolin K synthesis-related genes
mokA
,
mokB
,
mokE
, and
mokH
. Overall, these results suggest that
LaeA
plays a role in regulating the production of secondary metabolites and mycelial growth in
Monascus
. This study provides important insights into the mechanisms underlying the effects of the
LaeA
gene on the secondary metabolites of
M. purpureus
.