Science (American Association for the Advancement of Science), 2020-01, Vol.367 (6477)
2020
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- Autor(en) / Beteiligte
- Titel
- Monosomes actively translate synaptic mRNAs in neuronal processes
- Ist Teil von
- Science (American Association for the Advancement of Science), 2020-01, Vol.367 (6477)
- Ort / Verlag
- United States: The American Association for the Advancement of Science
- Erscheinungsjahr
- 2020
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- To accommodate their complex morphology, neurons localize messenger RNAs (mRNAs) and ribosomes near synapses to produce proteins locally. However, a relative paucity of polysomes (considered the active sites of translation) detected in electron micrographs of neuronal processes has suggested a limited capacity for local protein synthesis. In this study, we used polysome profiling together with ribosome footprinting of microdissected rodent synaptic regions to reveal a surprisingly high number of dendritic and/or axonal transcripts preferentially associated with monosomes (single ribosomes). Furthermore, the neuronal monosomes were in the process of active protein synthesis. Most mRNAs showed a similar translational status in the cell bodies and neurites, but some transcripts exhibited differential ribosome occupancy in the compartments. Monosome-preferring transcripts often encoded high-abundance synaptic proteins. Thus, monosome translation contributes to the local neuronal proteome.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.aay4991Titel-ID: cdi_proquest_miscellaneous_2350094856
- Format
- –
- Schlagworte
- Animals, Axonal plasticity, Axons, Brain slice preparation, Cell size, Compartments, Dendrites, Dendritic spines, Dendritic structure, Electron micrographs, Electrons, Elongation, Footprinting, Gene sequencing, Hippocampus, Hybridization, Information processing, Male, Mass spectrometry, Mass spectroscopy, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Morphology, Neurons, Neuropil, Neuropil - metabolism, Occupancy, Polypeptides, Polyribosomes, Polyribosomes - metabolism, Preferences, Presynapse, Protein Biosynthesis, Protein synthesis, Proteins, Proteome - metabolism, Proteomes, Proteomics, Ribosomes, Ribosomes - metabolism, RNA, Messenger - genetics, RNA, Messenger - metabolism, Rodents, Synapses, Synapses - metabolism, Synaptic plasticity, Transcription, Transcripts (Written Records), Translation