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Ultrastructure, molecular phylogeny, and prevalence rates of Alternosema bostrichidis gen. nov. sp. nov. (Microsporidia, Terresporidia), a parasite of Prostephanus truncatus and Dinoderus spp. (Coleoptera, Bostrichidae)
Ist Teil von
Parasitology research (1987), 2020-03, Vol.119 (3), p.915-923
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2020
Quelle
Springer LINK 全文期刊数据库
Beschreibungen/Notizen
A new species and a new genus of a microsporidium
Alternosema bostrichidis
isolated from an adult
Prostephanus truncatus
in Mexico and from three species of the genus
Dinoderus
in Nigeria are described. The microsporidium is monomorphic, monoxenic, and develops in direct contact with host cell cytoplasm. The infection first appears with thoracic muscles, followed by a generalized invasion of the host. All developmental stages are diplokaryotic. Sporogony is disporoblastic. Mature spores are ovoid. Unfixed spores measure 3.7–4.2 × 2.0–2.6 μm, fixed and stained spores 3.5–5.0 × 2.4–2.8 μm. The polaroplast consists of dense lamellae and rare lamellae. The polar tube is slightly anisofilar, consisting of 11–17 coils, with 9–14 proximal (130 nm in diameter) and 2–3 distal coils (120 nm in diameter) arranged in one layer. Molecular phylogenetic analysis based upon a short portion of small-subunit ribosomal RNA gene (Genbank accession # KP455651) placed the new microsporidium within
Liebermannia-Orthosomella
lineage, which contains multiple undescribed parasites. In particular,
A. bostrichidis
showed maximal sequence similarity of 95% to
Microsporidium
sp. BBRE2 (# FJ755987) from Baikalian
Diplacanthus brevispinus
(Amphipoda: Acanthogammaridae) and
Microsporidium
sp. Comp CD Van 2 (# KC111784) from compost and soil in Canada. Frequent, devastating epizootics of laboratory cultures of
A. bostrichidis
support its potential as a biological control agent of grain borers.