Details

Autor(en) / Beteiligte

• Lin, Qing
• Li, Yan
• Qu, Meng-Ke
• Liu, Xing
• Zhang, Zhi-Rong
• Zhang, Ling

Titel

Preliminary Investigation on Difference of Protein Compositions Between DC2.4 Cells and Their Derived Exosomes by nanoLC-MS/MS

Ist Teil von

• Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban, 2020-01, Vol.51 (1), p.81-86

Ort / Verlag

China

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• To preliminarily investigate the differences of protein composition between immature dendritic cells (DC2.4) and their derived exosomes (DC-Exo) using a relatively rapid and sample-saving method based on nano-flow liquid chromatography tandem mass spectrometry (nanoLC-MS/MS). The supernatant of DC2.4 cells culture medium was collected and gradient centrifugation was applied to primarily extract and isolate DC-Exo; then sucrose density gradient ultracentrifugation was adopted to purify the DC-Exo. Bradford protein assay was used to determine the total protein content of the purified DC-Exo, and dynamic light scattering and transmission electron microscope were conducted to characterize the morphology and size distribution of the DC-Exo. Afterwards, protein samples including DC2.4 cells and DC-Exo were prepared by FASP enzymolysis method. Samples were performed nanoLC-MS/MS assay. The μLPickUp sample loading mode was used and only 1 μg of protein sample was required for each assay. The phase of Transport liquid