The Plant journal : for cell and molecular biology, 2020-06, Vol.102 (5), p.931-947
2020
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Brassinosteroid‐mediated reactive oxygen species are essential for tapetum degradation and pollen fertility in tomato
- Ist Teil von
- The Plant journal : for cell and molecular biology, 2020-06, Vol.102 (5), p.931-947
- Ort / Verlag
- England: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2020
- Quelle
- Wiley-Blackwell Journals
- Beschreibungen/Notizen
- Summary Phytohormone brassinosteroids (BRs) are essential for plant growth and development, but the mechanisms of BR‐mediated pollen development remain largely unknown. In this study, we show that pollen viability, pollen germination and seed number decreased in the BR‐deficient mutant d^im, which has a lesion in the BR biosynthetic gene DWARF (DWF), and in the bzr1 mutant, which is deficient in BR signaling regulator BRASSINAZOLE RESISTANT 1 (BZR1), compared with those in wild‐type plants, whereas plants overexpressing DWF or BZR1 exhibited the opposite effects. Loss or gain of function in the DWF or BZR1 genes altered the timing of reactive oxygen species (ROS) production and programmed cell death (PCD) in tapetal cells, resulting in delayed or premature tapetal degeneration, respectively. Further analysis revealed that BZR1 could directly bind to the promoter of RESPIRATORY BURST OXIDASE HOMOLOG 1 (RBOH1), and that RBOH1‐mediated ROS promote pollen and seed development by triggering PCD and tapetal cell degradation. In contrast, the suppression of RBOH1 compromised BR signaling‐mediated ROS production and pollen development. These findings provide strong evidence that BZR1‐dependent ROS production plays a critical role in the BR‐mediated regulation of tapetal cell degeneration and pollen development in Solanum lycopersicum (tomato) plants. Significance Statement Brassinosteroid signaling regulator BZR1 directly regulates RBOH1 transcription, which mediates the production of ROS to trigger PCD and cell degradation in the tapetum of tomato and promotes pollen development.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0960-7412eISSN: 1365-313XDOI: 10.1111/tpj.14672Titel-ID: cdi_proquest_miscellaneous_2334263980
- Format
- –
- Schlagworte
- anther, Apoptosis, Apoptosis - genetics, Apoptosis - physiology, BRASSINAZOLE RESISTANT 1 (BZR1), Brassinosteroids, brassinosteroids (BRs), Brassinosteroids - metabolism, Cell death, Deficient mutant, Degeneration, Degradation, Fertility, Gene Expression Regulation, Plant - genetics, Gene Expression Regulation, Plant - physiology, Germination, Homology, Lycopersicon esculentum - metabolism, Lycopersicon esculentum - physiology, Plant growth, Plant Proteins - genetics, Plant Proteins - metabolism, Pollen, Pollen - metabolism, Pollen - physiology, pollen development, programmed cell death (PCD), Reactive oxygen species, reactive oxygen species (ROS), Reactive Oxygen Species - metabolism, Respiratory burst oxidase, RESPIRATORY BURST OXIDASE HOMOLOG 1 (RBOH1), Signal Transduction - genetics, Signal Transduction - physiology, Signaling, Solanum lycopersicum, Tomatoes