Details

Autor(en) / Beteiligte

• González‐Gutiérrez, Andrés
• Ibacache, Andrés
• Esparza, Andrés
• Barros, Luis Felipe
• Sierralta, Jimena

Titel

Neuronal lactate levels depend on glia‐derived lactate during high brain activity in Drosophila

Ist Teil von

• Glia, 2020-06, Vol.68 (6), p.1213-1227

Ort / Verlag

Hoboken, USA: John Wiley & Sons, Inc

Erscheinungsjahr

2020

Quelle

Access via Wiley Online Library

Beschreibungen/Notizen

• Lactate/pyruvate transport between glial cells and neurons is thought to play an important role in how brain cells sustain the high‐energy demand that neuronal activity requires. However, the in vivo mechanisms and characteristics that underlie the transport of monocarboxylates are poorly described. Here, we use Drosophila expressing genetically encoded FRET sensors to provide an ex vivo characterization of the transport of monocarboxylates in motor neurons and glial cells from the larval ventral nerve cord. We show that lactate/pyruvate transport in glial cells is coupled to protons and is more efficient than in neurons. Glial cells maintain higher levels of intracellular lactate generating a positive gradient toward neurons. Interestingly, during high neuronal activity, raised lactate in motor neurons is dependent on transfer from glial cells mediated in part by the previously described monocarboxylate transporter Chaski, providing support for in vivo glia‐to‐neuron lactate shuttling during neuronal activity. We characterize lactate/pyruvate transport in glial cells and neurons from Drosophila larval brain using FRET sensors. Raises in neuronal lactate during high neuronal activity is dependent on the transfer of monocarboxylates from glial cells.