Details

Autor(en) / Beteiligte

• Matsuda, Kyohei
• Sugawa, Mitsuhiro
• Yamagishi, Masahiko
• Kodera, Noriyuki
• Yajima, Junichiro

Titel

Visualizing dynamic actin cross‐linking processes driven by the actin‐binding protein anillin

Ist Teil von

• FEBS letters, 2020-04, Vol.594 (8), p.1237-1247

Ort / Verlag

England

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Anillin is a type of actin filament cross‐linking protein that stabilizes the actin‐based contractile ring during cytokinesis. To elucidate the underlying intermolecular interactions between actin filaments and anillin, we utilized total internal reflection fluorescence microscopy (TIRFM) and high‐speed atomic force microscopy (Hs‐AFM). Single‐molecule imaging of anillin using TIRFM showed that anillin exists as monomers with relatively low binding affinity for actin filaments. Real‐time imaging of actin filament cross‐linking dynamics induced by anillin using Hs‐AFM revealed that anillin monomers cross‐link with actin filaments at a distance of 8 nm and that the polarity of those filaments is both parallel and antiparallel. These results are consistent with anillin playing a role in actin ring transition in vivo, where it might be responsible for thinning the ring‐shaped apolar actin bundles.