Details

Autor(en) / Beteiligte

• Sánchez-Ortiz, M.
• Cruz, M.J.
• Sánchez-Díez, S.
• Villar, A.
• Ojanguren, I.
• Muñoz, X.

Titel

Immunomodulatory effect of pigeon serum in an acute and chronic murine model of bird fanciers lung

Ist Teil von

• Environmental research, 2020-03, Vol.182, p.108981-108981, Article 108981

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Since the immunopathological mechanisms of bird fancier’s lung (BFL) are not well known, we created two models of the disease (acute and chronic BFL) to study and compare the pathways involved in its immunopathogenesis. C57BL/6 mice were used. Two intraperitoneal injections of 100 μL of commercial pigeon serum (PS) or saline (SAL) were administered with an interval of 48 h in between. Subsequently, intranasal instillations of 40 μL of PS or SAL were performed three days a week, for three weeks in the acute model (AC/PS) and for twelve weeks in the chronic model (CR/PS). Total lung capacity (TLC) was assessed. Pulmonary inflammation was evaluated in bronchoalveolar lavage (BAL), and total serum immunoglobulin (Ig) G was measured in serum samples 24 h, 7 days and 14 days after the last exposure. Histological studies of lungs were assessed. A drop in TLC was observed in treated mice. This decrease was more marked in the CR/PS group (p < 0.001). Neutrophil and lymphocyte counts increased in both AC/PS and CR/PS groups (p < 0.01). The extent of airway inflammation was also examined in the histological analysis of the lungs, which showed predominant perivascular and peribronchiolar inflammation, with centrilobular oedema and subpleural inflammation in the AC/PS group. In the CR/PS group, the changes were greater, with increased levels of IL-5, IL-17F, IL-13 and IL-10 and decreased levels of IL-2. Bronchial inflammation is present in acute and chronic models of HP following exposure to PS. Our results support the role of neutrophils and IL-17 in the development of the disease and an evolution towards a Th-2 immune response in chronic HP. These models may serve as a tool for future studies of the pathogenesis of HP. •The immunopathological mechanisms of bird fancier’s lung are not well known.•We created two models of the disease (acute and chronic) to study the pathways involved in its immunopathogenesis.•The results support the role of neutrophils and IL-17 in the disease and a Th-2 immune response in the chronic form.•These models may serve as a tool for future studies of the pathogenesis of hypersensitivity pneumonitis.