Details

Autor(en) / Beteiligte

• Si, Wei
• Liu, Yanfang
• Xiao, Yuansheng
• Guo, Zhimou
• Jin, Gaowa
• Yan, Jingyu
• Shen, Aijin
• Zhou, Han
• Yang, Fan
• Liang, Xinmiao

Titel

An offline two-dimensional supercritical fluid chromatography × reversed phase liquid chromatography tandem quadrupole time-of-flight mass spectrometry system for comprehensive gangliosides profiling in swine brain extract

Ist Teil von

• Talanta (Oxford), 2020-02, Vol.208, p.120366-120366, Article 120366

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Gangliosides, widely distributed in tissues and body fluid, have been connected to the therapy of cancer and brain related diseases. The complexity of the gangliosides structures with different polar moieties coexisting, a carbohydrate moiety and a ceramide chain, make it a great challenge in separation and analysis science. This study aimed to develop a strategy on the basis of high-accuracy data collected by an offline two-dimensional (2D) supercritical fluid chromatography (SFC) × reversed phase liquid chromatography (RPLC)/quadrupole time-of-flight (Q-ToF) system, and to integrate an in-house library with self-developed software for fast screening and identification of gangliosides from a complex sample (swine brain extract). Subsequent positive-mode MS/MS was used to validate the identified gangliosides. Finally, 153 gangliosides were separated and 79 of them were identified by the in-house library and self-developed software, 4-fold more than those by manual identification (18 gangliosides). Among the identified ones, 20 were detected in swine brain for the first time. This study established an offline 2D SFC × RPLC system and provided a new method for fast screening and automatic identification of gangliosides in complex mixtures. It will be conducive to further study of biological functions of gangliosides. [Display omitted] •An offline SFC × RPLC two-dimensional system was constructed for GLS analysis for the first time.•A GLS fast screening and automatic identification strategy was established by in-house library with self-developed software.•79 gangliosides were tentatively identified in swine brain extract and 20 of them were new.