Details

Autor(en) / Beteiligte

• Li, Wenqiang
• Wu, Dongwei
• Hu, Duo
• Zhu, Shanshan
• Pan, Cile
• Jiao, Yanpeng
• Li, Lihua
• Luo, Binghong
• Zhou, Changren
• Lu, Lu

Titel

Stress-relaxing double-network hydrogel for chondrogenic differentiation of stem cells

Ist Teil von

• Materials Science & Engineering C, 2020-02, Vol.107, p.110333-110333, Article 110333

Ort / Verlag

Lausanne: Elsevier B.V

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• The mechanical environment of extracellular matrix (ECM) plays an important role in adjusting the behaviors of cells. Natural ECM are highly viscoelastic materials with stress-relaxion behavior. Hydrogel is considered as a promising and attractive material for cell carrier, but they are typically elastic serving as synthetic ECM. Double-network (DN) hydrogel has an interpenetrating network of special structure combining the advantages of both rigid and ductile components, due to which the mechanical properties of the system can be very different from that of the single-network ones, and some special biological properties can be obtained. In this study, GG/PEGDA DN hydrogel was prepared by combining gellan gum (GG) with polyethylene glycol diacrylate (PEGDA), and then the influence of the two individual networks on the viscoelasticity of the system were investigated. Furthermore, the effects of viscoelasticity of GG/PEGDA DN hydrogel on the biological behavior of bone mesenchymal stem cells (BMSCs) were explored in vitro and in vivo. The results indicate that the spreading of BMSCs was closely related to the relaxation behavior of the hydrogels. GG/PEGDA DN hydrogel shows excellent mechanical and relaxation properties which provide a favorable physical environment for cell proliferation and spreading, and induce chondrogenic differentiation. Our study demonstrates that this DN hydrogel has bright prospects in the fields of cell carrier and cartilage tissue engineering. [Display omitted] •Double network (DN) hydrogel was constructed with stress-relaxation behavior.•GG/PEGDA DN hydrogel is prominent in stress response and deformation recovery.•DN hydrogel and its parent single network ones own fairly different microstructures.•Cell spreading and proliferation can benefit from relaxation behavior of DN hydrogel.•DN hydrogel enhanced COL-II expression and accelerated chondrification in vivo.