Details

Autor(en) / Beteiligte

• Ferst, Juliana G.
• Rovani, Monique T.
• Dau, Andressa M.P.
• Gasperin, Bernardo G.
• Antoniazzi, Alfredo Q.
• Bordignon, Vilceu
• Oliveira, Dimas E.
• Gonçalves, Paulo B.D.
• Ferreira, Rogério

Titel

Activation of PPARG inhibits dominant follicle development in cattle

Ist Teil von

• Theriogenology, 2020-01, Vol.142, p.276-283

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The peroxisome proliferator-activated receptor gamma (PPARG, also called NR1C3) is a nuclear receptor of the peroxisome proliferator-activated receptor family (PPAR). PPARs are involved in the regulation of apoptosis, cell cycle, estradiol and progesterone synthesis, and metabolism. However, the role of PPARs and their regulation during follicular development and ovulation in monovular species remain poorly understood. In this study, a well-established intrafollicular injection model was used to investigate if the PPARG participates in the regulation of dominant follicle development and ovulation in cattle. Findings from this study revealed that the relative mRNA abundance of PPARG was similar between dominant and subordinate follicles around follicle deviation, decreased after the LH surge, and increased before ovulation. In addition, a quadratic correlation was found between PPARG mRNA levels in granulosa cells and progesterone concentration in the follicular fluid. Intrafollicular injection of 50 μM Troglitazone (TGZ; a PPARG agonist) inhibited follicular growth and decreased CYP19A1 mRNA abundance in granulosa cells. These findings indicate that PPARG is involved in the regulation of steroidogenesis, follicle growth and ovulation in cattle. •Activation of PPARG by synthetic agonist inhibit follicular growth in cattle.•Activation of PPARG decrease CYP19A1 mRNA abundance in granulosa cells.•mRNA abundance of PPARG decrease after the LH surge, and increase before ovulation.