Chemico-biological interactions, 2020-01, Vol.315, p.108893-108893, Article 108893
2020
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- Autor(en) / Beteiligte
- Titel
- Promotion of myotube differentiation and attenuation of muscle atrophy in murine C2C12 myoblast cells treated with teaghrelin
- Ist Teil von
- Chemico-biological interactions, 2020-01, Vol.315, p.108893-108893, Article 108893
- Ort / Verlag
- Ireland: Elsevier B.V
- Erscheinungsjahr
- 2020
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- This study aimed to investigate the effects of teaghrelin, an active ingredient of Chin-shin oolong tea, on murine C2C12 myoblast cells. Under high serum conditions, teaghrelin inhibited C2C12 cell proliferation, indicating a cell cycle arrest and cessation of proliferative progression. Teaghrelin promoted pro-differentiation of C2C12 cells as evidenced by a progressively elongated morphology, as well as the induction of muscle specific myogenin, myosin heavy chain (MHC), and MyoD. The formation of multinucleated myotubes, and the increase of MHC-positive immunoreactivity within the myotubes, further reflected a complete differentiation and maturation of the contractile skeletal muscle cells induced by teaghrelin. Like ghrelin, teaghrelin attenuated dexamethasone-decreased myotube diameter, indicating its protective effects against skeletal muscle atrophy. Additionally, the expressions of Atrogin-1 and MuRF-1 ubiquitin E3 ligase were reduced. In conclusion, the results highlight a possibility of developing teaghrelin as a functional food for the prevention or therapeutic treatment of disease-associated skeletal muscle atrophy. [Display omitted] •Teaghrelin attenuated C2C12 myoblast cell proliferation.•Teaghrelin promoted C2C12 cell differentiation and myotube formation.•Teaghrelin attenuated dexamethasone-induced myotube atrophy.•Teaghrelin attenuated dexamethasone-induced ubiquitin E3 ligase expression.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0009-2797eISSN: 1872-7786DOI: 10.1016/j.cbi.2019.108893Titel-ID: cdi_proquest_miscellaneous_2313653344
- Format
- –
- Schlagworte
- Animals, Cell Cycle Checkpoints - drug effects, Cell Differentiation - drug effects, Cell Line, Cell Proliferation - drug effects, Chin-shin oolong, Differentiation, Ghrelin, Ghrelin - pharmacology, Mice, Muscle atrophy, Muscle Fibers, Skeletal - drug effects, Muscle Fibers, Skeletal - metabolism, Muscle, Skeletal - drug effects, Muscle, Skeletal - metabolism, Muscular Atrophy - drug therapy, Muscular Atrophy - metabolism, Myoblasts - drug effects, Myoblasts - metabolism, MyoD Protein - metabolism, Myogenin - metabolism, Myosin Heavy Chains - metabolism, Plant Extracts - pharmacology, Protective Agents - pharmacology, Signal Transduction - drug effects, Tea - chemistry, Teaghrelin, Ubiquitin-Protein Ligases - metabolism