Blood, 2019-12, Vol.134 (25), p.2318-2329
2019
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Details
- Autor(en) / Beteiligte
- Titel
- Platelet lamellipodium formation is not required for thrombus formation and stability
- Ist Teil von
- Blood, 2019-12, Vol.134 (25), p.2318-2329
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- During platelet spreading, the actin cytoskeleton undergoes rapid rearrangement, forming filopodia and lamellipodia. Controversial data have been published on the role of lamellipodia in thrombus formation and stability. The Wiskott-Aldrich syndrome protein-family verprolin-homologous protein (WAVE)-regulatory complex, which has been shown in other cells to drive lamellipodium formation by enhancing actin nucleation via the actin-related protein 2/3 (Arp2/3) complex, is activated by Ras-related C3 botulinum toxin substrate 1 (Rac1) interaction with the WAVE complex subunit cytoplasmic fragile X mental retardation 1–interacting protein 1 (Cyfip1). We analyzed Cyfip1flox/floxPf4-Cre mice to investigate the role of Cyfip1 in platelet function. These mice displayed normal platelet counts and a slight reduction in platelet volume. Activation of mutant platelets was only moderately reduced to all tested agonists as measured by αIIbβ3 integrin activation and P-selectin surface exposure. However, lamellipodium formation of mutant platelets was completely abolished on different matrices. Nevertheless, Cyfip1-deficient platelets formed stable thrombi on collagen fibers ex vivo and in 2 models of occlusive arterial thrombosis in vivo. Similarly, the hemostatic function and maintenance of vascular integrity during inflammation of the skin and lung were unaltered in the mutant mice. Investigation of platelet morphology in an induced thrombus under flow revealed that platelets rather form filopodia in the thrombus shell, and are flattened with filopodium-like structures when in direct contact to collagen fibers at the bottom of the thrombus. We provide for the first time direct evidence that platelet lamellipodium formation is not required for stable thrombus formation, and that morphological changes of platelets differ between a static spreading assay and thrombus formation under flow. •Cyfip1 plays a crucial role for branching of actin filaments and for lamellipodium formation.•Lamellipodium formation is not required for the formation of a hemostatic plug or thrombus. [Display omitted]
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-4971eISSN: 1528-0020DOI: 10.1182/blood.2019002105Titel-ID: cdi_proquest_miscellaneous_2313359160
- Format
- –
- Schlagworte
- Actin-Related Protein 2-3 Complex - genetics, Actin-Related Protein 2-3 Complex - metabolism, Adaptor Proteins, Signal Transducing - genetics, Adaptor Proteins, Signal Transducing - metabolism, Animals, Blood Platelets - metabolism, Blood Platelets - pathology, Female, Male, Mice, Mice, Transgenic, Neuropeptides - genetics, Neuropeptides - metabolism, P-Selectin - genetics, P-Selectin - metabolism, Pseudopodia - genetics, Pseudopodia - metabolism, rac1 GTP-Binding Protein - genetics, rac1 GTP-Binding Protein - metabolism, Thrombosis - genetics, Thrombosis - metabolism, Thrombosis - pathology